Welcome to the 

Microbial Spatial Gene regulation Lab

 

Mission of the lab

Our research explores how the spatiotemporal control of gene expression shapes microbial cell physiology and differentiation

By integrating high-resolution imaging techniques, molecular biology and genetics, we investigate gene regulation in single fungal and bacterial cells, aming to understand how single cells adopt both individual and collective strategies to optimize their fitness

 We love microbes!

S. cerevisiae

Filamentous S. cerevisiae

Candida albicans

Arbuscular mycorrhizal fungi

Aspergiullus niger

E. coli

 We are always open to explore the physiology of new microorganisms. Contact us for collaborations!

1 - Methods development for RNA visualization and imaging analysis in fixed microbial cells based on single molecule RNA FISH (smFISH)

smFISH-IF in S. cerevisiae

Tutucci et al. 2020

2 - Methods development for RNA imaging in live microbial cells based on the MS2 and PP7 systems

Live imaging MS2 tagged mRNAMaekiniemi et al. 2024

 

3 - Regulation of filamentous fungi differentiation and biofilm formation

Candida albicans

4 - RNA localization and spatial control of protein synthesis

Schematic of S. cerevisiae bud 

mRNA localization

Das et. al 2021

5 - Regulation of mRNA degradation in S. cerevisiae

6 - Development of imaging analysis pipelines for microbial species

Questions?

Contact evelina.tutucci[at]vu.nl to get more information on the projects

Support: